bioB [2019-08-09 07:38:55]

biotin synthase

locus: BSU_30200

pI: 5.55

mw: 36.77 kDa

protein length: 335 aa

gene length: 1008 bp

function: biosynthesis of biotin

product: biotin synthase

essential: no

ec: 2.8.1.6

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]

The protein

Protein family

• [SW|Radical SAM superfamily] (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|1R30] (from ''E. coli'', 36% identity) [Pubmed|14704425]

Expression and Regulation

Operons

• genes: [gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
  description: [Pubmed|8763940,8892842]

  regulatory mechanism

  • [protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|8763940,8892842], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]

  regulation

  • repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]

Biological materials

Mutant

• BKE30200 ([gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGTTCCATCCATTGATTCA, downstream forward: _UP4_TGAAAGAATCAATAAAAGCA
• BKK30200 ([gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGTTCCATCCATTGATTCA, downstream forward: _UP4_TGAAAGAATCAATAAAAGCA

References

• 14704425,8763940,8892842